1.00 mL人体尿液样品经自制的萃取装置萃取后，收集萃取液，采用超高效液相色谱-串联质谱法快速测定萃取液中4种巯基尿酸的含量。以Waters Acquity UPLC^TM BEH C₁₈色谱柱为固定相，以不同体积比的0.05%（体积分数）乙酸溶液和甲醇的混合液为流动相进行梯度洗脱，串联质谱分析中采用电喷雾负离子源和多反应监测模式，采用内标法定量。4种巯基尿酸的检出限（3S/N）为0.012~0.082 μg·L^-1，测定下限（10S/N）为0.038~0.270 μg·L^-1。方法用于人体尿液样品的分析，日内回收率为93.9%~102%，日内相对标准偏差（n=7）为2.0%~3.4%，日间回收率为93.4%~102%，日间相对标准偏差（n=7）为2.4%~4.1%。

The sample of human urine (1.00 mL) was extracted by self-made extraction device. The extract was collected. UHPLC-MS/MS was applied to the rapid determination of 4 mercapturic acids in the extract. Waters Acquity UPLC^TM BEH C₁₈ chromatographic column was used as stationary phase, and the mixture of 0.05% (φ) acetic acid solution and methanol mixed in different ratios was used as mobile phase in gradient elution. ESI^- and multi-reactions monitor mode were adopted in MS/MS. Internal standard method was used for quantitative. Detection limits (3S/N) and lower limits of determination (10S/N) of the 4 mercapturic acids were in the ranges of 0.012-0.082 μg·L^-1 and 0.038-0.270 μg·L^-1 respectively. The proposed method was applied to the analysis of the sample of human urine. Values of recovery and RSDs (n=7) of intra-day were in the ranges of 93.9%-102% and 2.0%-3.4% respectively. Values of recovery and RSDs (n=7) of inter-day were in the ranges of 93.4%-102% and 2.4%-4.1% respectively.