Modification of the Standard Method for Determination of Sudan Red in Foodstuffs
标 签 高效液相色谱法 苏丹红染料 食品 正己烷 丙酮 HPLC Sudan red dyes Foodstuff n-Hexane Acetone
在高效液相色谱法(HPLC)测定食品中苏丹红染料的标准方法(GB/T 19681-2005)中规定试样经处理后溶于丙酮中,而苏丹红染料标准则用正己烷溶解.试验发现:苏丹红染料在上述两种不同的溶剂中进行HPLC测定时,同样浓度的染料得出的出峰时间和峰面积显著不同.按原标准的方法,由于试样及标准所用溶剂不同,造成定性及定量检测结果的明显偏差.因此各标准染料用正己烷溶解后须先转换成丙酮溶液方能用于制作标准曲线.经这一改进后,各染料在0.15~4.0 mg·L-1线性范围内的相关系数均大于0.999.测定值的相对标准偏差均小于5%,回收率达90%以上.
As described in the standard method(GB/T 19681-2005 HPLC Determination of Sudan Red Dyes in Foodstuffs),the sample is finally dissolved in acetone after pretreatment,and standards of the sudan red dyes are dissolved in n-hexane.It was found that in the HPLC determinations,significant differences of values of retention time tR and peak area were found for sudan red dyes of same concentration but in different solvents(e.g.in n-hexane or in acetone),and that remarkable deviations were obtained in both qualitative and quantitative testings as different solvents were used in dissolving sample and standards.As a modification,it was proposed that the n-hexane solutions of standard sudan red dyes should be converted to acetone solutions before they are used for preparation of standard curves.By this modification,the correlation coefficients obtained were over 0.999 for linearity range of 0.15-4.0 mg·L-1 of either the sudan red dyes,and values of RSD′s obtained were less than 5%,as well as values of recovery larger than 90%.
引用该论文: SHEN Bing,XI Qi-hui,YU Tian-xiang,YING Xiao-hong. Modification of the Standard Method for Determination of Sudan Red in Foodstuffs[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2008, 44(6): 535～537
沈兵,奚奇辉,于天祥,应晓虹. 对食品中苏丹红检测标准的改进[J]. 理化检验－化学分册, 2008, 44(6): 535～537