UHPLC-MS/MS Determination of Residual Amount of Enostrobilurin in Food
标 签 超高效液相色谱-串联质谱法 烯肟菌酯 食品 UHPLC-MS/MS Enostrobilurin Food
提出了用超高效液相色谱-串联质谱法测定食品中烯肟菌酯残留量的方法。样品经乙腈提取后,以伯仲胺、C18和无水硫酸镁为萃取剂用基质分散固相萃取净化,净化液经Eclipse Plus C18色谱柱(50 mm×2.1 mm,1.8 μm)分离,用乙腈与5 mmol·L-1乙酸铵溶液(75+25)的混合液洗脱,采用正离子模式监测。烯肟菌酯的质量浓度在50 μg·L-1以内与峰面积呈线性关系,检出限(3S/N)为1 μg·kg-1,以空白样品为基体进行加标回收试验,所得回收率在76.7%~89.6%之间,相对标准偏差(n=10)在4.2%~11%之间。
UHPLC-MS/MS was applied to determination of residual amount of enostrobilurin in food .The sample was extracted with acetonitrile and purified by dispersive solid phase extraction with primary secondary amine, C18 and anhydrous MgSO4 as extractants. The eluate obtained were used for UHPLC-MS/MS determination, in which Eclipse Plus C18 column (50 mm×2.1 mm,1.8 μm)was used as stationary phase, and a mixture of acetonitrile and 5 mmol·L-1 ammonium acetate (75+25) was used as mobile phase; positive electrospray ionization was used in the detection. Linear relationship between values of peak area and mass concentration of enostrobilurin was kept in the range within 50 μg·L-1, with detection limit (3S/N) of 1 μg·kg-1. On the base of blank sample, test for recovery was made by standard addition method; values of average recovery found were in the range of 76.7%-89.6%, with RSD′s (n=10) in the range of 4.2%-11%.
引用该论文: WU Jie-shan,CHEN Liang,FENG Jia-wang,REN Yong-xia,CAI Qin-ren. UHPLC-MS/MS Determination of Residual Amount of Enostrobilurin in Food[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2013, 49(8): 953～956
吴洁珊,陈亮,冯家望,任永霞,蔡勤仁. 超高效液相色谱-串联质谱法测定食品中烯肟菌酯残留量[J]. 理化检验－化学分册, 2013, 49(8): 953～956